DNA Helicase - unwinds the double helix by disrupting H bonds that bind the nitrogenous bases
Single-stranded binding proteins - prevent the separated DNA strands from re-attaching
RNA Primer - made up of multiple nitrogenous bases which are attached to the template strand and initiate the building of a new complementary strand i.e replication
Primase - builds RNA primers
DNA Polymerase I - removes RNA primers and replaces them with appropriate DNA nucleotides
DNA Polymerase III - creates complementary strands of DNA, attach to an RNA primer, and add nucleotides to the 3' end
DNA Ligase - joins Okazaki fragments together by phosphodiester bonds
DNA Gyrase - relieves tension produced when DNA double helix is unwound by helicase
