To disperse the inoculum, it is always best to utilize sterile equipment. After streaking each quadrant, flame the loop or use a fresh disposable loop.
By spreading the inoculum thinner and more uniformly, you may successfully dilute the inoculum on the plate and generate isolated colonies by using a fresh or sterilized loop.
Note that the options are neither given nor available, hence the general answer.
We can use the quadrant streaking approach to work with solitary or well-isolated colonies. It is a quick way of isolation. It gradually dilutes the microbial population number in the original sample.
Quadrant streaking is a technique used to distinguish organisms from mixed cultures.
The streak plate's objective is to isolate colonies from an inoculum by producing zones of increasing dilution on a single plate. Isolated colonies are clones of cells that evolved from a single progenitor cell.
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