Researchers want to use eDNA to look for an invasive species in a waterway.
Which step should they do last?
A. Cut the DNA into fragments of different sizes.
B. Compare the samples with a positive control.
C. Isolate DNA from the waterwakin question.
D. Stain the gel to reveal a banding pattern.

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mariahmtz2018 mariahmtz2018 · Answer 1 · 2020-11-05T21:03:29+01:00

the answer is A.
the researchers would not be able to compare the samples and cut the DNA when samples from the waterway are not collected.

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luisvaschetto luisvaschetto · Answer 2 · 2021-09-07T19:56:32+02:00

The last step to observe different DNA patterns is to stain the gel to reveal a banding pattern (Option D).

DNA extraction, polymerase chain reaction (PCR) and fragment separation by gel electrophoresis are basic molecular techniques used to identify patterns in DNA.

The patterns in DNA can be used for identifying an individual in a population, distinguishing different species, etc. These patterns can be obtained by using restriction enzymes.

There are different dyes used to stain DNA before the identification of patterns in DNA.

For example, ethidium bromide is a widely used dye to stain DNA before fragment separation by agarose gel electrophoresis.

In conclusion, the last step is TO STAIN the gel with a DNA dye to reveal a banding pattern (Option D is correct).

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