Answer: A double restriction digest
Explanation: A double restriction digest is a process were two restriction enzymes are used to digest DNA in a single reaction.in double restriction digest you will observe two bands,while in single restriction digest you will observe just one band. In double restriction digest no
Self - ligating plasmid because it create mismatch ends. It also aids in directional in section while single disgestion you have to screen your clones to be sure to get your plasmid with correct insert orientation.
