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. An inoculated thioglycolate medium culture tube is clear throughout the tube except for dense growth at the bottom of the tube. What is your conclusion?
The organisms are obligate anaerobes.
The organisms are facultative anaerobes.
The organisms are aerotolerant.
The organisms are obligate aerobes.

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Answer:

The organisms are obligate anaerobes.

Explanation:

  • At the bottom of the tube the level of oxygen is zero thats why the dense growth at the bottom of the tube show obligate anaerobic microorganisms.
  • At the middle of the tube micro-aerophilic microorganisms growth can be observed.
  • At the top of the tube obligate aerobic microorganisms will show their growth.
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