cwsmith206 cwsmith206

05-02-2020
Biology

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DNA from a newly discovered virus was purified, and UV light absorption was followed as the molecule was slowly heated. The absorbance increase at the melting temperature was only 10%. What does this result tell you about the structure of the viral DNA? Please be sure to make your initial post and respond to at least two classmates with additional questions and comments to further the discussion.

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olatunbosunadeoke olatunbosunadeoke

05-02-2020

Answer: The viral DNA has a sharp thermal transition.

Explanation:

Due to more guanine to cytosine (G-C) bonds in viral DNA, and the extra stability its presence confers, the double stranded Viral DNA is more stable to heat and have a higher melting point.

Hence, even when denatured by heat, the unstacked base pairs in its single strands only absorb ultraviolet light at a minimal rate such as 10% increase or less, before it gets renatured into double stranded DNA again.

Thus, the ability of viral DNA to adjust to thermal (heat) denaturation is the reason.

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