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You want to prepare 500mL of LB broth containing 100 µg/mL of ampicillin. Your stock solution of ampicillin is at a concentration of 100 mg/mL. How many microliters of the stock solution of ampicillin will you add to the 500 mL of LB broth? Round your answer to the nearest microliter.

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Answer:

500 µL

Explanation:

Total mass required of ampicillin:

  • 500 mL * 100 µg/mL= 50000 µg ampicillin
  • 50000 µg * 1mg/10³µg = 50 mg

The required volume V of the stock solution can be calculated by the formula:

Concentration * Volume = Mass

  • 100 mg/mL * V = 50 mg
  • V = 0.5 mL

Finally we convert mL into µL:

  • 0.5 mL * 10³µL/1mL = 500 µL

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