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The pKa of the α‑carboxyl group of serine is 2.21 , and the pKa of its α‑amino group is 9.15 . Calculate the average net charge on serine if it is in a solution that has a pH of 8.30 .

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Answer:

Net charge in serine at pH equal to 8.30 is "0"

Explanation:

  • At pH > [tex]pK_{a}[/tex], carboxyl group exists as [tex]-CO_{2}^{-}[/tex] (charged)
  • At pH < [tex]pK_{a}[/tex], carboxyl group exists as -COOH (neutral)
  • At pH > [tex]pK_{a}[/tex], amino group exists as [tex]-NH_{2}[/tex] (neutral)
  • At pH < [tex]pK_{a}[/tex], amino group exists as [tex]-NH_{3}^{+}[/tex] (charged)
  • So, at pH = 8.30, both carboxyl and amino group exists in charged state.
  • Net charge in serine at pH equal to 8.30 is "0".
  • Structure of serine at pH equal to 8.30 has been shown below.
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