You are cloning in linear DNA that contains the gene uiuC into PAMP. Answer the following questions. Use the plasmid map provided to you under Week 4 on the MCB 251 course website. Which of the following methods must be performed to obtain pure PAMP from a culture of E. coli?
a. DNA ligation
b. Restriction digestion
c. Bacterial transformation
d. QIAGEN miniprep
e. PCR