You think your digestion did not work because on your gel you see 3 bands while you only expected to see 1 band. What could these 3 bands be assuming you are correct that the digestion did not work?
A) Undigested vector, linearized vector, insert
B) Undigested vector, supercoiled vector, nicked vector
C) Insert, self-ligated vector, undigested vector
D) Supercoiled vector, linearized vector, insert