In a gene, an open reading frame (ORF) is the protein-coding sequence that begins with a start codon and ends with a stop codon.
Please examine the following two DNA sequences from both directions and predict the amino acid sequences of the protein translated from all possible ORFs of the mRNAs transcribed from the double-stranded DNA.
The answer for this problem is that each of the DNA sequence will transcribe into two different mRNA sequences and total of 6 reading frames for a DNA sequence. Each DNA strand can become a template strand. Can you explain how to find 6 reading frames?
DNA sequence 1:
5’GAGGCGATGCGCGATTGGAGCAAGGATCGATATCAGTGACGAGCTA-3’
3’CTCCGCTACGCGCTAACCTCGTTCCTAGCTATAGTCACTGCTCGAT-5’
DNA sequence 2:
5’GCGATCGTAGCTAGCGGCGACGACTAGCTAGCTGGATCGACATAGCA-3’
3’CGCTAGCATCGATCGCCGCTGCTGATCGATCGACCTAGCTGTATCGT-5’