What would happen to a PCR reaction if the following circumstances arose: the system failed to heat up, and it remained at room temperature for the entire duration?

A. The denaturation step would not occur, preventing DNA strand separation.
B. The annealing step would be prolonged, leading to increased nonspecific binding.
C. The extension step would not take place, hindering the synthesis of DNA strands.
D. The entire PCR process would be unaffected, and the reaction would proceed normally.