kirsten7164 kirsten7164

01-01-2024
Chemistry

contestada

How do you determine the annealing temperature of primers?
1) By using online tools to calculate the melting temperature
2) By performing a gradient PCR to find the ideal temperature
3) By following the formula: Tm = 2(A+T) + 4(G+C)
4) By consulting a scientific literature or protocol

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