Describe the construction of one of more synthetic operons on a single plasmid that can be transformed into E. coli and which will act as a sensor for AI-1 produced by Vibrio fischeri. When low levels of AI-1 are present, this strain will remain white. When high levels of AI-1 are present, strains containing this construct should turn purple/indigo. Please include a description of the plasmid you'll use as a cloning vector for this operon, and describe all genes necessary to produce the required phenotypes, and all required supplements to include in the media to enable the 0-Pcolor change. (Hint: wild type E. coli does not possess luxR).