A protein contains four disulfide bonds. In order to break these bonds researchers added a minimum of: A. 2 moles of NADH for each mole of protein. B. 4 moles of NADH for each mole of protein. C. 2 moles of NAD+ for each mole of protein. D. 4 moles of NAD+ for each mole of protein. Solution: The correct answer is B. This is correct. This Biochemistry question falls under the content category "Principles of chemical thermodynamics and kinetics." The answer is B because each mole of NADH can reduce a mole of disulfide bonds. Since the protein has four disulfide bonds, four moles of NADH are needed. This is a Scientific Reasoning and Problem Solving question because you must use the scientific model of NADH oxidation and disulfide reduction to make a prediction about molar equivalency.