In preparation for doing an experiment, you need to prepare a bu er solution with a pH of 7.8. A friend in your lab suggests using HEPES, a sulfonic acid with a pKa of 7.5. Since you know that the bu er pKa should be close to the desired pH, this sounds good. To make 500mL of a 0.5-M HEPES solution, you start by dissolving 0.25 moles of the acidic form of HEPES in about 300mL of water. This generates a slightly acidic solution, and you plan to adjust the pH to 7.8 by adding 1M NaOH. How much of this solution should you add

Q&A Education