Consider three different kinds of human libraries: a genomic library, a brain cDNA library, and a liver cDNA library.

a. Assuming inserts of approximately equal size, which would contain the greatest number of different clones?

b. Would you expect any of these libraries not to overlap the others at all in terms of the sequences it contains? Explain.

c. How do these three libraries differ in terms of the starting material for constructing the clones in the library?

d. Why would you need to sequence many clones from many cDNA libraries to annotate a genome?